Description
ELISA (Enzyme-Linked Immunosorbent Assay) is a highly sensitive and specific immunoassay platform for the quantitative determination of a wide range of analytes. Our ELISA kits utilise precise antibody-antigen interactions to deliver reliable and reproducible results.
General Specifications
Product Type: Enzyme-Linked Immunosorbent Assay (ELISA) Kits
Intended Use: For qualitative and/or quantitative detection of specific antigens, antibodies, or biomarkers in human serum, plasma, or other biological fluids. Suitable for clinical diagnostics and research applications
General Characteristics
Format: 96-well microplate (12 × 8 strips, breakable)
Assay Principle: Sandwich/indirect/competitive ELISA (parameter dependent)
Detection System: Horseradish peroxidase (HRP) with TMB chromogenic substrate
Specimen Type: Human serum, plasma or urine (depending on parameter)
Sample Volume: Typically 50–100 µl per well
Assay Time: 60–120 minutes (protocol dependent)
Readout: Optical density measurement at 450 nm (reference 620–630 nm)
Storage Condition: 2–8°C (do not freeze)
Shelf Life: 12–18 months from date of manufacture
Kit Size: 96 tests per kit (other formats available on request)
Performance Characteristics
Sensitivity: Optimised for low-level detection of analyte (parameter specific)
Specificity: High specificity through proprietary antibodies/antigens, minimal cross-reactivity
Precision: CV ≤10% intra- and inter-assay variation
Dynamic Range: Wide linear range suitable for clinical decision-making
Accuracy: Validated against gold-standard reference methods