Description
Spin column-based nucleic acid isolation kits offer a streamlined and efficient solution for isolating high-purity nucleic acids from diverse sample types.
General Specifications
Product Type: Nucleic Acid Extraction Kit (Silica Membrane Spin-Column Technology)
Intended Use: For rapid, reliable and cost-effective purification of high-quality genomic DNA and/or RNA from a wide variety of biological samples, suitable for downstream applications including PCR, RT-PCR, qPCR, sequencing, cloning and molecular diagnostics
General Characteristics
Extraction Principle: Silica membrane spin-column technology for selective binding of nucleic acids in the presence of chaotropic salts
Specimen Types: Whole blood, serum, plasma, swabs (nasopharyngeal/oropharyngeal), cultured cells, tissue lysates, urine, and viral transport medium (VTM)
Input Volume: Typically 50, 200 μl/sample (protocol dependent)
Elution Volume: 30–100 μl nuclease-free buffer or water
Processing Time: 30–40 minutes per batch (manual centrifugation or vacuum manifold)
Yield: High recovery with maximum nucleic acid retention
Purity: A260/A280 ratio ~1.8–2.0, suitable for PCR/qPCR applications
Throughput: Low-to-medium throughput; ideal for routine diagnostic and research laboratories
Storage Condition: Room temperature (15–30°C) for buffers and columns; enzymes (if provided) stored at 4°C
Shelf Life: 12–24 months from date of manufacture
Performance Characteristics
High Quality: Produces inhibitor-free nucleic acids suitable for sensitive downstream assays
Reproducibility: Consistent yields with minimal variation between runs
Scalability: Flexible sample input ranges, compatible with multiple manifolds
Sample Compatibility: Effective with both fresh and preserved samples
Formats Available:
- 50 prep kit
- 100 prep kit
- 200 prep kit
- Custom bulk packs for high-volume users