Description
RT-PCR is a gold-standard method for sensitive, specific nucleic acid amplification and quantification. Its applications span diagnostics, research, and beyond. We provide comprehensive RT-PCR solutions, empowering precise molecular analysis.
General Specifications
Product Type: Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR) Kit
Intended Use: For the qualitative and/or quantitative detection of specific RNA/DNA targets from clinical specimens (swabs, serum, plasma, body fluids) using real-time PCR platforms. Designed for use in clinical diagnostics and molecular research.
General Characteristics
Assay Principle: Reverse transcription followed by real-time amplification and detection using sequence-specific primers and hydrolysis/TaqMan probes (parameter dependent)
Target Specimens: Nasopharyngeal swab, oropharyngeal swab, serum, plasma, urine or VTM samples (as per intended application)
Detection Channel(s): Multiplexed fluorophores (e.g., FAM, HEX/VIC, ROX, Cy5, depending on target)
Internal Control: Exogenous or endogenous control included to monitor extraction quality and PCR inhibition
Sample Input Volume: Typically 5–10 µL of extracted nucleic acid
Reaction Volume: 20–25 µL
Assay Run Time: ~60–90 minutes (platform dependent)
Throughput: Compatible with 96-well and 384-well real-time PCR instruments
Storage Condition: –20 °C (avoid repeated freeze–thaw)
Shelf Life: 12–18 months from date of manufacture
Performance Characteristics
Sensitivity (LoD): As low as 1–100 copies per reaction (parameter specific)
Specificity: High target specificity with validated primer/probe design; no cross-reactivity with common pathogens
Reproducibility: CV ≤5% across replicates
Dynamic Range: 10² to 10⁸ copies/reaction (linear detection)
Accuracy: Validated against reference assays and international standards